Prashanth.Chiliveri
Bhagwan Mahavir Medical Research Centre, India
Title: A Study on the Association of TGF β1 (-509) and TNF- α (-308) Gene Polymorphisms with susceptibility to Rheumatic Heart Disease in South Indian Patients
Biography
Biography: Prashanth.Chiliveri
Abstract
Cytokines are the important mediators of inflammatory and immune responses to RF and RHD. The study aimed to investigate the association of TGF Beta -1 (-509) and TNF-Alpha (-308) gene polymorphisms with susceptibility to RHD. The study was carried out on 56 RHD and 45 ethnically matched healthy subjects for comparison (controls). Demographic data was collected and 2D echocardiography was carried out for confirmation of the disease. Blood samples were drawn from each subject, genomic DNA was isolated from each and subjected to PCR-RFLP to identify the polymorphisms of cytokine genes. Our results showed that GG genotype of TNF-Alpha (-308) was significantly associated with increased risk of RHD (P < 0.05, OR 3.1, CI 1.17-8.4) and G allele was found to be highly significant (P < 0.001, OR 2.81, CI 1.57 to 4.97). AG genotype showed 1.8 folds risk (P>0.05, 1.8, CI 0.65-5.1) and AG+GG combination showed 1.3 fold risk for RHD (P> 0.05 OR 1.3 CI 0.6-2.8). However, none of the genotypes of TGF-β- 1 showed an increase in the RHD patients compared to control subjects thus indicating that TGF-Beta-1 gene was not associated with susceptibility for RHD. Our findings suggest that TNF-Alpha (-308) gene polymorphism plays an important role in predisposition to RHD in Telangana population. However, our results showed that the TGF Beta- 1 (-509) was not associated with increased risk for RHD in this population.