Heart Diseases

Biography

Biography: Abhilasha Singh

Abstract

Overview:-Lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) is one of the major receptors for oxidized low-density lipoprotein (OxLDL) in the aorta of aged rats. OxLDL attenuates LOX-1 activation in endothelium of lipid accumulating sites of both animal and human model of hypercholesterolemia. This study has explored the binding affinity of Aegeline to LOX1 for the first time in comparison to Atorvastatin. Targeting LOX1 may provide novel diagnostic strategy for hypercholesterolemia and vascular diseases. Objective: This study was planned to address whether the possible effect of Aegeline on LOX-1 could be related to modulation of ox-LDL receptor in hypercholesterolemia. Methods: Thirty-six Wistar rats were divided into six groups. The pathology group rats were fed with HCD for 16 weeks and treatment groups rats were fed with HCD for 12 weeks and further supplemented with AG/AV for another 4 weeks. In vivo and in vitro experiments were carried out to find out the relative markers of the pathological condition likely Oxidized LDL (Ox-LDL) and LOX1 levels were determined. Standard biochemical parameters were carried out. Also, histopathological examination of aorta tissue sections was carried out. Oil red O staining was performed in IC21 macrophage cell lines. Docking studies were performed to find out the binding affinity of AG to LOX1. Results: AG administration effectively decreased the lipid levels induced by HCD as evidenced by lipid profile. Further, the lowered levels of Ox-LDL and LOX1 in AG administered rats deem it to be a potent anti-hypercholesterolemic agent. Compared to AV, AG had a pronounced effect in down- regulating the expression of lipids evidenced by Oil red O staining. AG binds with LOX1 at a higher affinity. Conclusion: This study thus validates AG to be an effective stratagem in bringing down the lipid stress induced by HCD and can be deemed as an anti-hypercholesterolemic agent.